C-TERMINAL FRAGMENTS OF PARATHYROID HORMONE-RELATED PROTEIN, PTHRP-(107-111) AND PTHRP-(107-139), AND THE N-TERMINAL PTHRP-(1-40) FRAGMENT STIMULATE MEMBRANE-ASSOCIATED PROTEIN-KINASE-C ACTIVITY IN RAT SPLEEN LYMPHOCYTES

Authors
WHITFIELD JF ISAACS RJ CHAKRAVARTHY BR DURKIN JP MORLEY P NEUGEBAUER W WILLIAMS RE WILLICK G RIXON RH
Citation
Jf. Whitfield et al., C-TERMINAL FRAGMENTS OF PARATHYROID HORMONE-RELATED PROTEIN, PTHRP-(107-111) AND PTHRP-(107-139), AND THE N-TERMINAL PTHRP-(1-40) FRAGMENT STIMULATE MEMBRANE-ASSOCIATED PROTEIN-KINASE-C ACTIVITY IN RAT SPLEEN LYMPHOCYTES, Journal of cellular physiology, 158(3), 1994, pp. 518-522
Citations number
38
Categorie Soggetti
Physiology,"Cytology & Histology
Journal title
Journal of cellular physiology → ACNP
ISSN journal
00219541
Volume
158
Issue
3
Year of publication
1994
Pages
518 - 522
Database
ISI
SICI code
0021-9541(1994)158:3<518:CFOPHP>2.0.ZU;2-S
Abstract
Membrane-associated protein kinase C (PKC) activity in lymphocytes fre shly isolated from rat spleen was stimulated by the C-terminal parathy roid hormone-related protein fragments, PTHrP-(107-111) and PTHrP-(107 -139), at concentrations from 10(-3) to 10(4) (P)M. By contrast, the s ame concentrations of PTHrP-(l20-139), without the 107-111 TRSAW (-Thr -Arg-Ser-Ala-Trp-) sequence of the other C terminal fragments, did not stimulate spleen lymphocyte PKC. Low concentrations of the N-terminal PTHrP-(1-40) fragment also stimulated membrane-associated PKC activit y in the spleen lymphocytes. These results suggest that PTHrP might be an important physiological regulator of the immune response. Publishe d 1994 Wiley-Liss, Inc.