PURIFICATION OF ANTIBODY-ANTIGEN COMPLEXES CONTAINING RECOMBINANT SIVPROTEINS - COMPARISON OF ANTIGEN AND ANTIBODY-ANTIGEN COMPLEXES FOR IMMUNE PRIMING

Authors
RANDALL RE YOUNG D HANKE T SZAWLOWSKI P BOTTING C
Citation
Re. Randall et al., PURIFICATION OF ANTIBODY-ANTIGEN COMPLEXES CONTAINING RECOMBINANT SIVPROTEINS - COMPARISON OF ANTIGEN AND ANTIBODY-ANTIGEN COMPLEXES FOR IMMUNE PRIMING, Vaccine, 12(4), 1994, pp. 351-358
Citations number
29
Categorie Soggetti
Immunology
Journal title
Vaccine → ACNP
ISSN journal
0264410X
Volume
12
Issue
4
Year of publication
1994
Pages
351 - 358
Database
ISI
SICI code
0264-410X(1994)12:4<351:POACCR>2.0.ZU;2-F
Abstract
This paper describes a general procedure for the two-step purification of recombinant proteins as antibody-antigen complexes in which there is no uncomplexed antibody or antigen. In this way, immune complexes c ontaining the p17, p27, vpr and vpx proteins of simian immunodeficienc y virus (SIV) have been purified. Antibody-antigen complexes are more immunogenic than antigen when administered either alone or with alum. The significance of the work is that this general method could be modi fied for the manufacture of immune complexes for incorporation into mu ltivalent vaccines.