PURIFICATION AND CHARACTERIZATION OF PECTINESTERASE PRODUCED BY A STRAIN OF ASPERGILLUS-NIGER

Authors
MALDONADO MC DESAAD AMS CALLIERI D
Citation
Mc. Maldonado et al., PURIFICATION AND CHARACTERIZATION OF PECTINESTERASE PRODUCED BY A STRAIN OF ASPERGILLUS-NIGER, Current microbiology, 28(4), 1994, pp. 193-196
Citations number
17
Categorie Soggetti
Microbiology
Journal title
Current microbiology → ACNP
ISSN journal
03438651
Volume
28
Issue
4
Year of publication
1994
Pages
193 - 196
Database
ISI
SICI code
0343-8651(1994)28:4<193:PACOPP>2.0.ZU;2-U
Abstract
After an 88-fold purification, pectinesterase produced by a strain of Aspergillus niger, isolated from rotten lemons, showed the following m ain characteristics: maximum activity at 45 degrees C, pH 5; Km, with pectin as substrate, 1.01 mg/L; Delta G(), 4750 Cal/mol. Polygalactur onic acid and methanol acted as competitive and non-competitive inhibi tors, respectively. The activity of the enzyme was impaired by MgCl2 a nd stimulated by NaCl.