M. Clerici et al., ROLE OF INTERLEUKIN-10 IN T-HELPER CELL DYSFUNCTION IN ASYMPTOMATIC INDIVIDUALS INFECTED WITH THE HUMAN-IMMUNODEFICIENCY-VIRUS, The Journal of clinical investigation, 93(2), 1994, pp. 768-775
The loss of T helper cell (TH) function in asymptomatic HIV type 1-inf
ected individuals occurs before the decline in CD4(+) T cells. At leas
t part of the loss in TH function results from changes in immunoregula
tory cytokine profiles. To investigate the role of IL-10 in such dysre
gulation, we tested whether: (a) expression of IL-10-specific mRNA wou
ld be upregulated in PBMC from asymptomatic, HIV-infected (HIV+) indiv
iduals; (b) PBMC from these same individuals would produce increased l
evels of IL-10 when stimulated in vitro with phytohemagglutinin; and (
c) defective antigen-specific TH function could be restored by anti-IL
-10 antibody. We observed that IL-10-specific mRNA was marginally upre
gulated, and increased levels of IL-10 were produced by PBMC from HIV individuals compared with PBMC from uninfected individuals, Those ind
ividuals whose TH function was more severely compromised produced high
er levels of IL-10. Additionally, defective antigen-specific TH functi
on in vitro could be reversed by anti-IL-10 antibody, including the re
sponse to HIV envelope synthetic peptides. Furthermore, the antigen-sp
ecific TH responses of HIV-uninfected PBMC could be reduced with IL-10
, a process reversed by anti-IL-10. These results confirm that the ear
ly loss of TH function in HIV+ individuals is due at least in part to
cytokine-induced immune dysregulation, and support the hypothesis of a
switch from a predominant type 1 state to a predominant type 2 condit
ion in HIV infection.