GROWTH AND PRODUCTION OF RIFAMYCIN OXIDASE BY CURVULARIA-LUNATA

Curvularia lunata could neither grow nor produce rifamycin oxidase in synthetic media without peptone and yeast extract. Mycelia grown on co mplex media were tested for the ability to produce rifamycin oxidase i n synthetic media. The optimum concentrations of peptone and past extr act were in the range of 75-10 g/L. Five percent inoculum size was fou nd to be optimum for good growth and enzyme production. Addition of me tal ions to the cultivation medium increased the enzyme activity.