EXTERNAL CONDITIONS INFLUENCING ETHANOL OXIDATION BY RESTING CELLS OFCANDIDA-UTILIS

Effects of ethanol inhibition, initial pH and buffering capacity of me dia on the catabolic activity of nongrowing cells of Candida utilis we re studied. Effects of external conditions on the kinetic of ethanol o xidation and cell respiration are described by mathematical models. Th e results revealed a significant influence of both the external pH and the buffering capacity of the medium on the kinetic parameters of cat abolic activity. The inhibitory effect results in the bottleneck of on e of the reaction of the citrate cycle, glyoxylate cycle or electron t ransfer in a respiratory chain although the total rate of ethanol diss imilation increases under these conditions.