ALCOHOLYSIS OF EPSILON-DECALACTONE WITH POLYETHYLENE GLYCOL-MODIFIED LIPASE IN 1,1,1-TRICHLOROETHANE

Authors
FURUKAWA M KODERA Y UEMURA T HIROTO M MATSUSHIMA A KUNO H MATSUSHITA H INADA Y
Citation
M. Furukawa et al., ALCOHOLYSIS OF EPSILON-DECALACTONE WITH POLYETHYLENE GLYCOL-MODIFIED LIPASE IN 1,1,1-TRICHLOROETHANE, Biochemical and biophysical research communications, 199(1), 1994, pp. 41-45
Citations number
10
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
199
Issue
1
Year of publication
1994
Pages
41 - 45
Database
ISI
SICI code
0006-291X(1994)199:1<41:AOEWPG>2.0.ZU;2-U
Abstract
Lipase from Pseudomonas cepacia was modified with 2,4-bis[O-methoxypol y(ethylene glycol)]-6-chloro-s-triazine, activated PEG2, to form PEG-l ipase. The PEG-lipase is soluble and active in organic solvents. It ca talyzes alcoholysis of racemic epsilon-decalactone with ethanol in 1,1 ,1 -trichloroethane to form (R)hydroxydecanoic acid ethyl ester. No al coholysis of (S)-decalactone takes place. These results were discussed in relation to carbon number of n-alcohol, optimum temperature and co mparison with modified and non-modified lipases. (C) 1994 Academic Pre ss, Inc.