A. Baracca et al., DIETARY LIPIDS AND 5'-NUCLEOTIDASE ACTIVITY OF RAT-CELL PLASMA-MEMBRANES, Biochemical and biophysical research communications, 199(1), 1994, pp. 99-105
Three groups of adult rats were fed black currant or olive oils or a 1
:1 mixture of the two for three months. Feeding black currant oil diet
s, high in 18:2 (n-6), 18:3 (n-6), 18:3 (n-3), increased the heart and
liver plasma membrane content of linoleic and arachidonic acid with a
concomitant decrease of oleic acid. PUFA, n-3 and n-6 content and the
bilayer lipid fluidity as examined by measuring the fluorescence anis
otropy of diphenylhexatriene were not significantly affected. On the c
ontrary, the 5'-nucleotidase of liver membrane of rats fed black curra
nt diets was lower than that observed in membranes of liver from olive
oil fed rats. Therefore it is concluded that PUFA and n-3/n-6 ratio a
s well as membrane fluidity do not influence the 5'-nucleotidase activ
ity. It is suggested that the activity is sensitive to the amount of a
specific fatty acid of the membrane (i.e., oleic or arachidonic acid)
and/or to lipid supplementation which can influence the eicosanoids m
etabolism. (C) 1994 Academic Press, Inc.