A protease (Df-protease) from house dust mites (Dermatophagoides farin
ae), which is closely related to mite-induced allergic diseases, catal
yzes the activation of kallikrein-kinin system in plasma (3, 6, 12). D
f-protease was competitively inhibited by guanidine and amidine deriva
tives with low-molecular weight. The kinetic study revealed that the K
i values of 1-[p-(p-amidinophenoxycarbonyl)benzoyl]-D-pro benzyl ester
acetate and -methoxycarbonyl-4-amidinophenoxycarbonyl)benzoyl] -N-phe
nylglycine ethyl ester acetate were 5 x 10(-9) M and 6 x 10(-9) M, res
pectively.