INHIBITION OF A MITE PROTEASE (DF-PROTEASE) BY SYNTHETIC INHIBITORS

Authors
MATSUSHIMA A SHIOYA K KOBAYASHI M NOGUCHI Y NAKAI H KODERA Y INADA Y
Citation
A. Matsushima et al., INHIBITION OF A MITE PROTEASE (DF-PROTEASE) BY SYNTHETIC INHIBITORS, Biomedical research, 15(1), 1994, pp. 55-58
Citations number
13
Categorie Soggetti
Medicine, Research & Experimental
Journal title
Biomedical research → ACNP
ISSN journal
03886107
Volume
15
Issue
1
Year of publication
1994
Pages
55 - 58
Database
ISI
SICI code
0388-6107(1994)15:1<55:IOAMP(>2.0.ZU;2-O
Abstract
A protease (Df-protease) from house dust mites (Dermatophagoides farin ae), which is closely related to mite-induced allergic diseases, catal yzes the activation of kallikrein-kinin system in plasma (3, 6, 12). D f-protease was competitively inhibited by guanidine and amidine deriva tives with low-molecular weight. The kinetic study revealed that the K i values of 1-[p-(p-amidinophenoxycarbonyl)benzoyl]-D-pro benzyl ester acetate and -methoxycarbonyl-4-amidinophenoxycarbonyl)benzoyl] -N-phe nylglycine ethyl ester acetate were 5 x 10(-9) M and 6 x 10(-9) M, res pectively.