D. Sarrouilhe et al., SPECIFICITY OF RAT-LIVER PLASMA-MEMBRANE SERINE THREONINE PROTEIN-KINASES AND PHOSPHATASES OVER ENDOGENOUS PROTEINS, Cellular and molecular biology, 40(2), 1994, pp. 123-127
The specificity of rat liver plasma membrane protein kinases and phosp
hatases was examined over endogenous substrates, using specific effect
ors of these enzymes. cAMP-dependent protein kinase was shown to phosp
horylate the 77, 60 and 51 kDa phosphoproteins and type II casein kina
se, a specific 24 kDa one. On the contrary, types 1 and 2A protein pho
sphatases seemed to have a broad specificity in plasma membranes. An a
nalysis of the phosphoprotein pattern based on the endogenous substrat
es of plasma membrane enzymes was deduced from these and other results
from our laboratory. The specificity of some enzymes might arise from
the anchorage in plasma membrane which might restrict their activity
to their immediate environment.