C. Melguizo et al., ACTINOMYCIN-D CAUSES MULTIDRUG-RESISTANCE AND DIFFERENTIATION IN A HUMAN RHABDOMYOSARCOMA CELL-LINE, Cellular and molecular biology, 40(2), 1994, pp. 137-145
The emergence of drug-resistant tumor cells remains a major problem in
cancer chemotherapy. Resistance to multiple unrelated antineoplastic
drugs may be related, in part, to expression of the P-glycoprotein. Th
e cell line RD, derived from an embryonic rhabdomyosarcoma tumor, was
used as an in vitro model to examine the development of drug resistanc
e. A cell line resistant to actinomycin D (RD-DAC) was developed by gr
owing RD in increasing concentrations of the drug. The ID50 (concentra
tion of drug needed to induce a 50% reduction in cell growth) of the r
esultant line to actinomycin D was more than 15 times that of the pare
ntal line. The resistant line was cross-resistant to vincristine and d
oxorubicin. Resistance to actinomycin D resulted in increased P-glycop
rotein expression, which was associated with a change in desmin and vi
mentin expression. These results suggest that exposure to chemotherape
utic drugs can induce not only classical multidrug resistance, but als
o a process of cellular differentiation in rhabdomyosarcoma cells.