IDENTIFICATION AND SEQUENCE-ANALYSIS OF THE REGION ENCODING THE SITE-SPECIFIC INTEGRATION SYSTEM FROM LEUCONOSTOC-OENOS (OENOCOCCUS-OENI) TEMPERATE BACTERIOPHAGE-PHI-10MC

Citation
E. Gindreau et al., IDENTIFICATION AND SEQUENCE-ANALYSIS OF THE REGION ENCODING THE SITE-SPECIFIC INTEGRATION SYSTEM FROM LEUCONOSTOC-OENOS (OENOCOCCUS-OENI) TEMPERATE BACTERIOPHAGE-PHI-10MC, FEMS microbiology letters, 147(2), 1997, pp. 279-285
Citations number
17
Categorie Soggetti
Microbiology
Journal title
ISSN journal
03781097
Volume
147
Issue
2
Year of publication
1997
Pages
279 - 285
Database
ISI
SICI code
0378-1097(1997)147:2<279:IASOTR>2.0.ZU;2-G
Abstract
Malolactic fermentation conducted by Leuconostoc oenos is an essential step in winemaking. L. oenos bacteriophages are thought to be respons ible for fermentation failures, yet they have received little attentio n. The integration system of bacteriophage phi 10MC in the LOF111 L. o enos strain chromosome was studied and a 1456 bp phage DNA fragment wa s cloned and sequenced. An open reading frame (int) showing homology w ith several temperate bacteriophage integrases was located upstream of the phage attachment site (attP). This organisation is comparable to other phage site-specific recombination systems. The same bacterial at tachment site (attB) located in a tRNA(leu) gene was found in all 15 L . oenos strains studied and was involved in phi 10MC integration in th e bacterial chromosome.