IDENTIFICATION AND SEQUENCE-ANALYSIS OF THE REGION ENCODING THE SITE-SPECIFIC INTEGRATION SYSTEM FROM LEUCONOSTOC-OENOS (OENOCOCCUS-OENI) TEMPERATE BACTERIOPHAGE-PHI-10MC
E. Gindreau et al., IDENTIFICATION AND SEQUENCE-ANALYSIS OF THE REGION ENCODING THE SITE-SPECIFIC INTEGRATION SYSTEM FROM LEUCONOSTOC-OENOS (OENOCOCCUS-OENI) TEMPERATE BACTERIOPHAGE-PHI-10MC, FEMS microbiology letters, 147(2), 1997, pp. 279-285
Malolactic fermentation conducted by Leuconostoc oenos is an essential
step in winemaking. L. oenos bacteriophages are thought to be respons
ible for fermentation failures, yet they have received little attentio
n. The integration system of bacteriophage phi 10MC in the LOF111 L. o
enos strain chromosome was studied and a 1456 bp phage DNA fragment wa
s cloned and sequenced. An open reading frame (int) showing homology w
ith several temperate bacteriophage integrases was located upstream of
the phage attachment site (attP). This organisation is comparable to
other phage site-specific recombination systems. The same bacterial at
tachment site (attB) located in a tRNA(leu) gene was found in all 15 L
. oenos strains studied and was involved in phi 10MC integration in th
e bacterial chromosome.