ISOLATION AND PARTIAL CHARACTERIZATION OF CRISPACIN-A, A CELL-ASSOCIATED BACTERIOCIN PRODUCED BY LACTOBACILLUS-CRISPATUS JCM-2009

Authors
TAHARA T KANATANI K
Citation
T. Tahara et K. Kanatani, ISOLATION AND PARTIAL CHARACTERIZATION OF CRISPACIN-A, A CELL-ASSOCIATED BACTERIOCIN PRODUCED BY LACTOBACILLUS-CRISPATUS JCM-2009, FEMS microbiology letters, 147(2), 1997, pp. 287-290
Citations number
11
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
147
Issue
2
Year of publication
1997
Pages
287 - 290
Database
ISI
SICI code
0378-1097(1997)147:2<287:IAPCOC>2.0.ZU;2-U
Abstract
Crispacin A, a cell-associated bacteriocin produced by Lactobacillus c rispatus JCM 2009, was purified from culture broth by ammonium sulfate precipitation, followed by ion exchange and reversed-phase chromatogr aphy. Crispacin A was also purified from the cells of L. crispatus JCM 2009 by acid extraction and reversed-phase chromatography. Purified c rispacin A was determined to be 5393 Da by mass spectrometry and found not to show sequence homology with other bacteriocins from lactic aci d bacteria.