CHARACTERIZATION OF TRIACYLGLYCEROLS IN SATURATED LIPID MIXTURES WITHAPPLICATION TO SALATRIM 23CA

SALATRIM 23CA is a member of the SALATRIM family of triacylglycerols d eveloped by RJR Nabisco Co. It is an interesterification product of tr iacetin, tripropionin, and hydrogenated canola oil. To characterize th is sample, a combination of reversed-phase high-performance liquid chr omatography and high-temperature capillary gas chromatography (HTCGC) in sequence was used to separate the SALATRIM 23CA into individual com ponents. An on-line coupling of HTCGC with positive chemical ionizatio n mass spectrometry revealed the structure of the individual component s. To obtain detailed quantitative information, a combination of a 5-m OV-1 capillary column and a 25-m methyl 65% phenyl silicone Al-clad c olumn was used to determine the quantities of acylglycerols. A total o f 55 components were characterized in this study. They included 52 tri acylglycerols and 3 diacylglycerols.