GENETIC TOXICOLOGY STUDIES OF SALATRIM STRUCTURED TRIACYLGLYCEROLS .2. LACK OF GENETIC-DAMAGE IN IN-VITRO MAMMALIAN-CELL ASSAYS AND THE IN-VIVO MICRONUCLEUS ASSAY
Jr. Hayes et al., GENETIC TOXICOLOGY STUDIES OF SALATRIM STRUCTURED TRIACYLGLYCEROLS .2. LACK OF GENETIC-DAMAGE IN IN-VITRO MAMMALIAN-CELL ASSAYS AND THE IN-VIVO MICRONUCLEUS ASSAY, Journal of agricultural and food chemistry, 42(2), 1994, pp. 521-527
The SALATRIM family of triacylglycerols differs from other fats in the
ratio of short-chain fatty acids (SCFA) to long-chain fatty acids (LC
FA) and in that stearic acid is the major LCFA. These fats have calori
c availability values (4.5-6 kcal/g) lower than that of corn oil (9 kc
al/g). SALATRIM 23CA Lot A014, a typical SALATRIM fat,:was tested in i
n vitro mammalian cell genotoxicity assays including the chromosomal a
berration, unscheduled DNA: synthesis, and HPRT mammalian cell mutagen
esis assays. Corn oil also was tested as a reference fat. Both the SAL
ATRIM fat and corn oil were negative in the three assays. SALATRIM 234
CA lot A019 and SALATRIM 234CS lot A018 were tested in the in vivo bon
e marrow micronucleus assay. Rats received these SALATRIM fats or corn
oil at 10% (W/W) in the diet for 13 weeks. Both fats were negative in
this assay. The data confirm the prediction that SALATRIM fats lack g
enotoxic potential.