INHERITANCE OF ISOENZYMES AND THEIR LINKAGE RELATIONSHIPS IN 2 INTERSPECIFIC CHERRY PROGENIES

Two interspecific cherry progenies, Prunus avium 'Napoleon' x P. incis a E621 and x 19 nipponica F1292, were analysed by polyacrylamide gel e lectrophoresis for 14 enzyme systems: aconitase (AGO), acid phosphatas e (ACP), alcohol dehydrogenase (ADH), amylase (AMY), glutamate oxaloac etate transaminase (GOT), glucose-6-phosphate isomerase (GPI), isocitr ate dehydrogenase (ADH), leucine aminopeptidase (LAP), malate dehydrog enase (MDH), malic enzyme (ME), phosphogluconate dehydrogenase (PGD), phosphoglucomutase (PGM), shikimate dehydrogenase (SKD) and superoxide dismutase (SOD). Thirty-one loci were deduced from segregating bandin g patterns, Aco-2, Acp-1 to -5, Acp-8, -9, Adh-1 to -6, Amy-2, -3, Got -1 to -3, Gpi-2, IDd-1 to -4, Lap-1, Me-1, -2, Mdh-2, Pgd-1, -2 and So d-2. Only ten of these had previously been established. Seven putative loci were polymorphic but did not segregate in the progenies. Analysi s of cosegregations and calculation of recombination fractions reveale d that 15 loci could be grouped into four linkage groups: Acp-1/-2/-3- Acp-5; Gpi-2-Got-2- Got-1-Lap-1; Adh-4/-6-Amy-2; and Adh-1-Adh-5-Adh-2 -Me-2. These consolidate two previously reported linkage groups and es tablish three new groups. The previously reported linkage of Lap-1 wit h Me-1 was not confirmed. Fourteen cultivars of I! avium were analysed for the same 14 enzyme systems and showed polymorphism for just 17 of the established loci and for none of the putative loci, indicating fa r less scope for linkage analysis in intraspecific progenies from cros ses among these cultivars.