BROWN PIGMENTATION OF XANTHOMONAS-CAMPESTRIS PV PHASEOLI ASSOCIATED WITH HOMOGENTISIC ACID

The diffusible brown pigment produced by some strains of Xanthomonas c ampestris pv. phaseoli was found to be due to the secretion and subseq uent oxidation of homogentisic acid (2,5-dihydroxyphenylacetic acid) r ather than tyrosinase activity as previously reported. Homogentisic ac id is an intermediate in tyrosine catabolism for a number of bacteria. Brown-pigmented strains appeared to be disrupted in tyrosine cataboli sm and could not use tyrosine as a nutrient. Nonpigmented strains, how ever, could utilize tyrosine as a nutrient, and although they secreted homogentisic acid, the levels were approximately 1/100th that of brow n-pigmented strains. Production of brown pigment was stimulated by tyr osine and repressed by glucose. Growth in glucose resulted in a drop i n the pH of the media, and the greatest pigment formation was associat ed with a shift to alkaline conditions in the culture media. By buffer ing the media near pH 7.0, brown pigment formation was repressed, even though unoxidized homogentisic acid accumulated in the culture media. The disruption of tyrosine catabolism corresponds to other reports de scribing differences between brown-pigmented and nonpigmented strains, and provides further support for the retention of a special taxonomic status for the brown-pigmented strains.