Winter hexaploid wheat (Triticum aestivum L.) was crossed with Aegilop
s variabilis to transfer resistance to powdery mildew into wheat. Foll
owing two backcrosses to wheat and from 5 to 9 generations of selfing,
several disomic addition and substitution lines of hexaploid wheat re
sistant to the mildew pathogen were isolated. A pair of short satellit
ed chromosomes was always observed in the resistant lines. Further evi
dence utilizing as markers for homoeologous group 1 HMW glutenin subun
its and DNA hybridization with probe pGBX 3076 showed that an alien su
bstitution involved this homoeologous group.