PRODUCTION OF HUMAN PARATHYROID-HORMONE BY RECOMBINANT ESCHERICHIA-COLI TG1 ON SYNTHETIC MEDIUM

Production of human parathyroid hormone (hPTH) by Escherichia coli TG1 :I52cIts was studied. The hPTH is expressed as a fusion protein under control of the bacteriophage lambdap(R) promoter. The organism grows o n glucose/mineral salt medium and the expression of the gene product w as investigated under variation of temperature and growth rate prior t o and after induction. hPTH formation largely depends on cultivation t emperature and is optimal for a temperature shift from 30 to 38-degree s-C. Product expression is growth coupled and specific hPTH concentrat ion is independent of growth rate. The results are compared with a pre vious study on E. coli N4830:pEX-PPTH grown on complex media.