HAPTEN SYNTHESIS AND IMMUNOASSAY DEVELOPMENT FOR THE ANALYSIS OF CHLORODIAMINO-S-TRIAZINE IN TREATED PESTICIDE WASTE AND RINSATE

A significant environmental degradation product of chloro-s-triazine h erbicides is chlorodiamino-s-triazine. Current chromatographic analyti cal methods are limited by the water solubility and inefficient recove ry of this analyte. A diamino-s-triazine hapten was synthesized for th e production of polyclonal antibodies. Other haptens were synthesized which differed in bridging group and ring substitution for use in immu noassay development using both indirect and haptenated enzyme formats. In general, antibody recognition of substituted s-triazines decreased as a function of amino side chain substitution. The assays were sensi tive in the low micromolar range. Two assays were optimized and valida ted by comparison with a HPLC method for the analysis of chlorodiamino -s-triazine in treated pesticide waste samples. The correlation coeffi cients found were 0.988 and 0.979. It is anticipated that this immunoa ssay will provide a useful monitoring technique for on-site sampling a nd remediation of pesticide-contaminated areas.