ANTIBODY PREVALENCE OF HOG-CHOLERA, AUJES ZKYS-DISEASE AND THE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN WILD BOAR IN THE FEDERAL STATES OF SACHSEN-ANHALT AND BRANDENBURG (GERMANY)

During the hunting season from 1991/1992 blood samples were collected from wild the Federal States of Sachsen-Anhalt (482 samples) and Brand enburg (177 samples) which corresponds to 2.1 and 0.4 % of the total h unting bag. All sera were screened in a complex trapping blocking (CTB ) ELISA for antibodies against hog cholera virus (HCV) and in an indir ect ELISA for antibodies against Aujeszky's disease virus (ADV). Addit ionally the sera were tested for neutralizing antibodies against HCV s train ALFORT/187, bovine viral diarrhoea virus (BVDV) strains NADL and 1138/69, and against an ADV field isolate. In case of questionable re sults sera were tested against HCV strain ''BERGEN'', HCV vaccine stra in ''RIEMS'' and three HCV field isolates from wild boar. The serologi cal testing for antibodies against ''porcine reproductive and respirat ory syndrome virus'' (PRRSV) was carried out in indirect immunoperoxid ase monolayer assay (IPMA). Four sera (Sachsen-Anhalt) reacted positiv e in CTB-ELISA. Seven sera yielded neutralizing antibodies against HCV but only one of the ''non-negative'' samples scored positive in both techniques, ELISA and VNT. Two sera (Brandenburg) had low neutralizing antibody titres against Alfort/ 197 but scored negative in CTB-ELISA. Screening for antibodies against ADV of 640 sera led to 13 positive s era including 5 positive findinas in both ELISA and VNT