FIELD-EVALUATION OF 2 COLORIMETRIC COLIPHAGE DETECTION METHODS

Two new methods for coliphage detection, a colorimetric agar-based (CA B) method and a liquid colorimetric presence-absence (LCPA) method, we re compared to the coliphage method proposed by the American Public He alth Association (APHA; Standard Methods for the Examination of Water and Wasterwater, 18th ed., American Public Health Association, Washing ton, D.C., 1992). Both new methods are based on the induction of beta- galactosidase in Escherichia coli and the release of the enzyme throug h a lytic cell infection. The released enzyme then cleaves a chromogen ic substrate which produces a colored reaction product. Ninety split w ater samples from four different sources were tested. A total of 52 sa mples were positive by the CAB method, 52 were positive by the LCPA me thod, and 53 were positive by the APHA method. Results indicated that (i) the CAB and LCPA methods were as sensitive in coliphage detection as the APHA method, (ii) both the CAB and LCPA methods were easier to read and interpret than the APHA method, and (iii) the CAB method dete cted more coliphages in a positive sample than the APHA method in two of the four types of water sources. Importantly, the rapid and simple LCPA method was as reliable and sensitive as either of the two agar-ba sed methods in coliphage detection.