A METHOD TO DETECT ENTEROVIRUSES IN SEWAGE SLUDGE-AMENDED SOIL USING THE PCR

Authors
STRAUB TM PEPPER IL ABBASZADEGAN M GERBA CP
Citation
Tm. Straub et al., A METHOD TO DETECT ENTEROVIRUSES IN SEWAGE SLUDGE-AMENDED SOIL USING THE PCR, Applied and environmental microbiology, 60(3), 1994, pp. 1014-1017
Citations number
12
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
3
Year of publication
1994
Pages
1014 - 1017
Database
ISI
SICI code
0099-2240(1994)60:3<1014:AMTDEI>2.0.ZU;2-V
Abstract
PCR detection of seeded poliovirus type 1 in sludge-amended soil was m ade possible by utilizing Sephadex G-50 and Chelex-100 resins to remov e compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested slud ge were detected by PCR in 10 different soils amended with this sludge .