M. Stratz et al., PLASMID TRANSFER INTO THE HOMOACETOGEN ACETOBACTERIUM-WOODII BY ELECTROPORATION AND CONJUGATION, Applied and environmental microbiology, 60(3), 1994, pp. 1033-1037
Shuttle vectors (pMS3 and pMS4) which replicated in Escherichia coli a
nd in gram-positive Acetobacterium woodii were constructed by ligating
the replication origin of plasmid pAM beta 1 with the E. coli cloning
vector pUC19 and the tetM gene of streptococcal transposon Tn916. Ele
ctrotransformation of A. woodii was achieved at frequencies of 4.5 x 1
0(3) transformants per mu g of plasmid DNA. For conjugal plasmid trans
fer, the mobilizable shuttle vector pKV12 was constructed by cloning t
he tetM gene into pAT187. Mating of E. coli containing pKV12 with A. w
oodii resulted in transfer frequencies of 3 x 10(-6) to 7 X 10(-6) per
donor or recipient.