OPTIMIZATION OF THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY FOR VIRGINIAMYCIN DETERMINATION IN MINERAL PREMIXES

Authors
BLAIN P DEMAESSCHALCK E VANROMPAEY I GOSSELE F
Citation
P. Blain et al., OPTIMIZATION OF THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY FOR VIRGINIAMYCIN DETERMINATION IN MINERAL PREMIXES, Analyst, 119(2), 1994, pp. 361-364
Citations number
6
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst → ACNP
ISSN journal
00032654
Volume
119
Issue
2
Year of publication
1994
Pages
361 - 364
Database
ISI
SICI code
0003-2654(1994)119:2<361:OOTHLA>2.0.ZU;2-G
Abstract
An optimized reversed phase high-performance liquid chromatography met hod for the determination of virginiamycin in mineral premixes is pres ented. It consists of an extraction of the active components with a mi xture of acetone and citric acid (0.5 mol l(-1)), supplemented with 2% thioacetamide, followed by centrifugation or filtration and dilution in acetonitrile-water (1 + 1). The validation shows that the method is reproducible, precise, repeatable and linear between 5 and 50 mu g ml (-1) of virginiamycin activity.