THE EFFECT OF INTERLEUKIN-1-BETA (IL-1-BETA) ON THE REGULATION OF IL-1 RECEPTOR-TYPE-I MESSENGER-RIBONUCLEIC-ACID AND PROTEIN-LEVELS IN CULTURED HUMAN ENDOMETRIAL STROMAL AND GLANDULAR CELLS
C. Simon et al., THE EFFECT OF INTERLEUKIN-1-BETA (IL-1-BETA) ON THE REGULATION OF IL-1 RECEPTOR-TYPE-I MESSENGER-RIBONUCLEIC-ACID AND PROTEIN-LEVELS IN CULTURED HUMAN ENDOMETRIAL STROMAL AND GLANDULAR CELLS, The Journal of clinical endocrinology and metabolism, 78(3), 1994, pp. 675-682
Because we hypothesize that the interleukin-1 (IL-1) system may be imp
ortant in the dialogue between mother and embryo during the implantati
on process, we have analyzed the effect of IL-1 beta, a secretory prod
uct of the human embryo and human endometrium, on the mRNA and protein
levels of IL-1 receptor type I (IL-1R tI) in the human endometrium. F
or this purpose, endometrial epithelial cells (EEC) and stromal cells
(ESC) were isolated and cultured with progesterone (3.18 mu g/mL) and
epidermal growth factor (20 ng/mL) for 8 days in the presence or absen
ce of hrIL-1 beta (20 pg/mL). EEC from proliferative and secretory end
ometrium expressed high levels of IL-1R tI mRNA compared to ESC, and t
hese levels were not modulated by IL-1 beta. However, prostaglandin E(
2) levels peaked on day 4 in EEC treated with progesterone, epidermal
growth factor, and IL-1 beta (208.7 +/- 92 ng/10(7) cells), whereas no
prostaglandin E(2) was detectable in cells not treated with IL-1 beta
, indicating that these cells responded to IL-1 beta. With regard to E
SC from secretory endometrium, IL-1 beta increased its own receptor mR
NA levels (4 +/- 0.5-fold increase) after 8 days in culture. However,
when ESC were isolated from proliferative endometrium, an up-regulatio
n of IL-1R tI (3.5 +/- 0.5-fold increase) was observed on days 6 and 8
of culture regardless of the presence or absence of IL-1 beta. Immuno
reactive IL-1R tI was identified in cultured EEC and ESC, and patterns
similar to those of mRNA were observed. The constitutive presence of
IL-1R tI in EEC, which was not affected by IL-1 beta, and the up-regul
ation of IL-1R tI mRNA by its ligand IL-1 beta in ESC isolated during
the luteal phase suggest a role for the IL-1 system in human implantat
ion.