ON THE ORIGIN OF SPANISH 2-ROWED BARLEYS

To investigate the phylogenetic origin of Spanish two-rowed barleys, w e studied 44 accessions of old land-races both morphologically and bio chemically to ascertain their similarity with 51 entries of old cultiv ars and land-races of widespread origin across Europe. They were also compared with 20 accessions of Hordeum spontaneum from the Mediterrane an basin and other regions of its distribution range, 14 accessions of Moroccan cultivated six-rowed barley landraces, and different six-row ed Spanish and two-and six-rowed European cultivars. CM- (trypsin inhi bitors and subunits of the barley tetrameric alpha-amylase inhibitor) proteins and hordeins, all of which are endosperm proteins, were used as biochemical markers. The appearance of separate clusters of the Spa nish barleys in the numerical classifications for both protein systems as a result of the existence of characteristic gene combinations that do not exist in entries from other origins permitted us to postulate the existence of local ancestors for most of the Spanish two-rowed bar leys studied, and, therefore, a possible in situ domestication.