PURIFICATION AND ANTIGENICITY OF SALMONELLA-NEWPORT ENTEROTOXIN

The enterotoxic moiety present in cell free culture supernatant (CFCS) of S. newport strains (MP/120 and BM/704) was purified and antigenica lly characterised. Purification was achieved to homogeneity by salt pr ecipitation, dialysis and successive gel filtration through Sephadex G -100 and G-200 columns with the help of FPLC set. It was non-dialysabl e and purified enterotoxin yielded a single protein band in PAGE. It a ppeared to be of high molecular weight (100 KDa) and highly immunogeni c in the rabbit. Antigenically, it was not related to cholera toxin, S higa toxin or heat-labile enterotoxin of Escherichia coli.