A. Rudat et R. Ehwald, INDUCTION OF BETACYANIN FORMATION IN CHENOPODIUM-ALBUM CELL-CULTURES BY COCULTIVATION WITH THE DUCKWEED WOLFFIA-ARRHIZA, Plant cell reports, 13(5), 1994, pp. 291-294
Cells of Chenopodium album and whole plants of the duckweed Wolffia ar
rhiza were cocultivated. In the presence of Wolffia arrhiza the synthe
sis of a red-violet pigment (betacyanin) was induced in several cells
or cell clusters of Chenopodium album in the light. The exchange of so
lutes through the liquid phase was necessary for the induction of pigm
ent formation. The red-violet cells could be selected and subcultivate
d resulting in a red callus. A reddish cell suspension was obtained in
liquid culture in the presence of the duckweeds.