R. Kelsh et al., HOMEOTIC GENE-EXPRESSION IN THE LOCUST SCHISTOCERCA - AN ANTIBODY THAT DETECTS CONSERVED EPITOPES IN ULTRABITHORAX AND ABDOMINAL-A PROTEINS, Developmental genetics, 15(1), 1994, pp. 19-31
To investigate what role homeotic genes may play in morphological evol
ution, we are comparing homeotic gene expression in two very different
insects, Drosophila (Diptera) and Schistocerca (Orthoptera). In this
paper we describe a monoclonal antibody, FP6.87, that recognizes the p
roducts of both the Ultrabithorax (Ubx) and abdominal-A (abd-A) genes
in Drosophila, via an epitope common to the carboxy terminal region of
these two proteins. This antibody recognizes nuclear antigens present
in the posterior thorax and abdomen of Schistocerca. We infer that it
recognizes the Schistocerca homolog of UBX protein, and probably also
of ABD-A. As the distribution of Schistocerca ABD-A protein is alread
y known, we can use this reagent to map the expression of Schistocerca
UBX in the thorax and anterior abdomen, where ABD-A is not expressed.
Both the general domain, and many of the details, of UBX expression a
re remarkably conserved compared with Drosophila. Thus UBX expression
extends back from T2 in the ectoderm (including the CNS), but only fro
m Al in the mesoderm. As noted for other bithorax complex genes in Sch
istocerca, expression begins in the abdomen, at or shortly before the
time of segmentation. It only later spreads anteriorly to the thorax.
For much of embryogenesis, the expression of UBX in the thoracic epide
rmis is largely restricted to the T3 limb. In this limb, UBX is striki
ngly regulated, in a complex pattern that reflects limb segmentation.
Reviewing these and earlier observations, we conclude that evolutionar
y changes affect both the precise regulation of homeotic genes within
segments, and probably also the spectrum of downstream genes that resp
ond to homeotic gene expression in a given tissue. Overall domains of
homeotic gene expression appear to be well conserved between different
insect groups, though a change in the extent and timing of homeotic g
ene expression may underlie the modification of the posterior abdomen
in different insect groups. (C) 1994 Wiley-Liss, inc.