METHOD FOR THE ACCURATE MEASUREMENT OF FREEZING-INDUCED DENATURATION OF OVALBUMIN WITH 5,5'-DITHIOBIS-(2-NITROBENZOIC ACID)

Authors
WATANABE T KITABATAKE N DOI E
Citation
T. Watanabe et al., METHOD FOR THE ACCURATE MEASUREMENT OF FREEZING-INDUCED DENATURATION OF OVALBUMIN WITH 5,5'-DITHIOBIS-(2-NITROBENZOIC ACID), Bioscience, biotechnology, and biochemistry, 58(2), 1994, pp. 359-362
Citations number
17
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
58
Issue
2
Year of publication
1994
Pages
359 - 362
Database
ISI
SICI code
0916-8451(1994)58:2<359:MFTAMO>2.0.ZU;2-O
Abstract
A new method for measuring the freezing denaturation of ovalbumin with 5,5'-dithiobis-(2-nit robenzoic acid) (DTNB) is described. One ml of an ovalbumin solution (0.1%) containing 50 mu M DTNB at pH 7.0 was sub jected to 5 freeze-thaw ((FT) cycles. After centrifugation of the solu tion, the absorption at 412 nm was measured as an index of denaturatio n. This method was applied to study the cryoprotective effect of sucro se. The method offers a simple and accurate means for measuring the de naturation of ovalbumin produced by FT cycles and for measuring the ef fect of cryoprotectants on this denaturation.