EFFECTS OF PYRIDOXAL 5'-PHOSPHATE ON THE REFOLDING OF ASPARTATE-AMINOTRANSFERASE

Authors
YOSHIMURA T WAKAYAMA M KIM DW ESAKI N SODA K
Citation
T. Yoshimura et al., EFFECTS OF PYRIDOXAL 5'-PHOSPHATE ON THE REFOLDING OF ASPARTATE-AMINOTRANSFERASE, Bioscience, biotechnology, and biochemistry, 58(2), 1994, pp. 363-365
Citations number
12
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
58
Issue
2
Year of publication
1994
Pages
363 - 365
Database
ISI
SICI code
0916-8451(1994)58:2<363:EOP5OT>2.0.ZU;2-A
Abstract
The effects of pyridoxal 5'-phosphate on the refolding of aspartate am inotransferase were studied. The refolding of the cytoplasmic isozyme of pig heart aspartate aminotransferase denatured with 6M guanidine hy drochloride depends critically on the presence of pyridoxal 5'-phospha te. Pyridoxal 5'-phosphate affects a refolding intermediate at guanidi ne hydrochloride concentrations between 3.5 and 2.0 M to bring out the correct folding. In contrast, thermostable aspartate aminotransferase of Bacillus sp. YM-2 can be refolded independently of pyridoxal 5'-ph osphate.