COMPARISON OF PH-MEMBRANE ENZYME ELECTRODES FOR UREA WITH COVALENTLY BOUND ENZYME

Authors
KONCKI R KOPCZEWSKA E GLAB S
Citation
R. Koncki et al., COMPARISON OF PH-MEMBRANE ENZYME ELECTRODES FOR UREA WITH COVALENTLY BOUND ENZYME, Analytical letters, 27(3), 1994, pp. 475-486
Citations number
21
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
27
Issue
3
Year of publication
1994
Pages
475 - 486
Database
ISI
SICI code
0003-2719(1994)27:3<475:COPEEF>2.0.ZU;2-M
Abstract
pH-membrane electrode with tridodecylamine as a hydrogen-ionophore was used for construction of urea biosensors. Carboxylated PVC membrane m atrix allowed covalent enzyme binding to its surface. Several methods of chemical enzyme immobilization were tested. Urea enzyme electrodes with extended life-time (about 2 months) were obtained. The sensors ch aracteristics are in good agreement with theoretical expectations. The influence of the immobilization method on the stability of the prepar ed biosensors is discussed.