A. Bustos et al., INHIBITION OF HISTONE ANTI-HISTONE REACTIVITY BY HISTONE-BINDING SERUM COMPONENTS - DIFFERENTIAL EFFECT ON ANTI-H1 VERSUS ANTI-H2B ANTIBODIES/, Clinical and experimental immunology, 95(3), 1994, pp. 408-414
IgG fractions were purified on a protein G-agarose column from sera of
both systemic lupus erythematosus (SLE) patients and healthy donors.
All IgG fractions, after elution with 0-5 M acetic acid, reacted with
histones in an anti-histone ELISA assay, and IgG anti-histone activity
was in all instances higher in the IgG fraction than in the correspon
ding whole serum. This was shown to be due to the presence in serum of
histone-binding components that inhibited IgG binding to histones. Bo
th normal human and SLE patients' sera had these histone-binding compo
nents, and disparity between serum-positive and -negative anti-histone
antibody (AHA) tests was not dependent on differences in the blocking
capacity but on IgG antibody levels and avidity. Interaction of norma
l serum IgG fraction with all five histones was of low avidity, wherea
s interaction of IgG from AHA-positive SLE sera with both H1 and H2B h
ad high avidity. Low-affinity antibodies to every histone fraction, bu
t also high-affinity anti-H1 antibodies, were preferentially inhibited
. Our data indicate that several serum protein components are inhibiti
ng histone/anti-histone interaction and may play a protective role aga
inst both high-affinity anti-H1 antibodies present in SLE patients, an
d natural, low-affinity, anti-histone antibodies. As some acute phase
proteins, notably C-reactive protein, bind to histones, it is conceiva
ble that they play such a role. High-affinity anti-H2B antibodies, pre
sent in some SLE patients, and not inhibited by these serum components
, may, on the other hand, participate in the pathogenesis of the disea
se.