ANTIGENIC CROSS-REACTIVITY BETWEEN HUMAN T-LYMPHOTROPIC VIRUS TYPE-I (HTLV-I) AND RETINAL ANTIGENS RECOGNIZED BY T-CELLS

To investigate the relationship between uveitis and HTLV-I infection, we examined the cross-reactivity between HTLV-I antigens and retinal a ntigens recognized by T cells in B10.BR mice immunized with human HTLV -I-infected MT-2 cells. We found that T cells obtained from MT-2 immun e mouse spleen responded not only to HTLV-I antigens but also to retin al antigens of various species. However, they did not respond to HTLV- I-negative lymphoid cell lines. Furthermore, established T cell lines from MT-2 immune spleen cells also responded to both HTLV-I and retina l antigens. The phenotype of the immune cells that responded to both H TLV-I and retinal antigens was CD4(+), CD8(-), and CD3(+). The prolife rative response of T cell lines to HTLV-I as well as various retinal a ntigens, was clearly blocked by addition of anti-CD3, anti-CD4, or ant i-I-A(k) MoAbs, but not by anti-CD8 antibody. The established T cell l ines from HTLV-I immune spleen cells were all found to be CD3(+), TCR beta(+), CD4(+) CD8(-) cells by flow cytometric analysis. These result s indicate that an epitope of HTLV-I antigens Is cross-reactive to an epitope of retinal antigens extracted from either human retinoblastoma or normal murine, rat, and bovine retinae at a T cell recognition lev el.