DEPENDENCE OF CHOLECYSTOKININ-8-STIMULATED INSULIN RELEASE ON HIGH GLUCOSE-LEVELS IS EVIDENCED BY PSEUDO-ALPHA-D-GLUCOSE IN RAT PANCREAS AND ISLETS

The glucose-dependency of cholecystokinin-8 (CCK-8)-stimulated insulin release was investigated at high (11.1 and 16.7 mM) glucose concentra tions in rat pancreas perfusion and islet perifusion using glucose ana logues, pseudo-alpha-D-glucose and 2-deoxy-D-glucose. In perfused panc reas, both glucose analogues (22.4 mM) inhibited high glucose (16.7 mM )-induced insulin release, but not normal glucose (5.6 mM)-induced ins ulin release, with or without CCK-8 (1 nM). In perifused islets, the s ame level of either of the glucose analogues inhibited high glucose (1 1.1 mM)-induced insulin release, with or without CCK-8 (100 nM). These results demonstrate that CCK-8-stimulated insulin release only at hig h glucose level is glucose-dependent.