ANTI-IL-10 ANTIBODY ENHANCES THE PROLIFERATION OF CD8(-CELL CLONES - AUTOREGULATORY ROLE OF MURINE IL-10 IN CD8(+) T-CELLS() T)

All CD8(+) T cell clones used in this study secreted IL-10, and their proliferation was inhibited by exogenous IL-10. This study addresses t he question of whether IL-IO produced by responding T cell clones woul d inhibit proliferation of the secreting T cells themselves. Anti-IL-1 0 antibodies enhanced the proliferative response of the CD8(+) T cell clones, the enhancing effect appearing in the late period of prolifera tion. However, the proliferation of both CD4(+) Th1 and Th2 clones was not affected by anti-IL-10 throughout the culture. In addition to tha t of the cloned T cells, the proliferative response of a primary cultu re of CD8(+) T cells was enhanced by the anti-IL-10 antibody. This is the first report on a lymphokine which has an autoregulatory role in i nhibiting T cell proliferation. (C) 1994 Academic Press, Inc.