A COMPARISON OF THE ALLELIC FREQUENCIES OF 10 DNA POLYMORPHISMS ASSOCIATED WITH FACTOR-VIII AND FACTOR-IX GENES IN THAI AND WESTERN-EUROPEAN POPULATIONS
Ac. Goodeve et al., A COMPARISON OF THE ALLELIC FREQUENCIES OF 10 DNA POLYMORPHISMS ASSOCIATED WITH FACTOR-VIII AND FACTOR-IX GENES IN THAI AND WESTERN-EUROPEAN POPULATIONS, Blood coagulation & fibrinolysis, 5(1), 1994, pp. 29-35
The frequency of five factor VIII gene intragenic and linked DNA polym
orphisms and five factor IX gene intragenic polymorphisms was studied
in Thai females. The polymorphisms in the FVIII gene were detected by
restriction enzymes BclI, XbaI, BglI. and at linked loci DX13 (DXS15)
and St14 (DXS52) by BglII and TaqI, respectively, and in the FIX gene
by MseI, DdeI, XmnI, TaqI and HhaI. With the exception of the BglI res
triction fragment length polymorphism (RFLP), which is absent in Thais
, factor VIII polymorphism frequencies were similar in Thais and Cauca
sians. Combined use of XbaI and TaqI/St14 resulted in a heterozygosity
rate of greater than 90% in Thai females. For FIX, the recently descr
ibed MseI RFLP in the 5' flanking region was the most informative poly
morphism in Thais, 43% of females being heterozygous. The other four p
olymorphisms added little to the overall heterozygosity rate. The appr
opriate polymorphisms were used to track defective factor VIII and IX
genes through 22 Thai pedigrees with haemophilia to enable carrier sta
tus to be assigned to female family members. The information obtained
during this study will form the basis for carrier detection and prenat
al diagnosis of haemophilia A and B by DNA polymorphism analysis in Th
ailand.