Semen from 4 wild-caught giant pandas (Ailuropoda melanoleuca) held at
the China Conservation and Research Centre for the Giant Panda was co
llected (22 samples during 1991-1993) by electroejaculation, and evalu
ated for use in artificial insemination. Semen characteristics (mean /- SD) recorded were as follows: semen volume -1.5 +/- .9 ml (range-0.
3-3.5); sperm density 1.5 +/- 0.1 x 10(9)/ml (range-0.24-4.2); motilit
y 79 +/- 10% (range-60-95); abnormal sperm 14 +/- 5% (range-10-27); an
d pH 7.1 +/- 0.2 (range-6.7-7.5). There were significant differences f
rom year to year (P < 0.05) in semen volume collected and in the perce
ntage abnormal sperm in 1993 compared to other years. There were no si
gnificant differences among semen produced from the four different pan
das. Data collected were similar to reports for other giant pandas, an
d semen from all 4 giant pandas was considered suitable for use in art
ificial insemination. (C) 1994 Wiley-Liss, Inc.