The feasibility of applying ecologically valid and socially relevant e
motional stimuli in a standardized fashion to obtain reliable mood cha
nges in healthy subjects was examined. The stimuli consisted of happy
and sad facial expressions varying in intensity. Two mood-induction pr
ocedures (happy and sad, each consisting of 40 slides) were administer
ed to 24 young healthy subjects, who were instructed to look at each s
lide (self-paced) and try to feel the happy or sad mood expressed by t
he person in the picture. On an emotional self-rating scale, subjects
rated themselves as relatively happier during the happy mood-induction
condition and as relatively sadder during the sad mood-induction cond
ition. Conversely, they reported that they were less happy during the
sad mood-induction condition and less sad during the happy mood-induct
ion condition. The effects were generalized to positive and negative a
ffect as measured by the Positive and Negative Affect Scale. The intra
individual variability in the effect was very small. In a retest study
after 1 month, the mood-induction effects showed good stability over
time. The results encourage the use of this mood-induction procedure a
s a neurobehavioral probe in physiologic neuroimaging studies for inve
stigating the neural substrates of emotional experience.