Ct. Harker et al., COOLING AUGMENTS HUMAN SAPHENOUS-VEIN REACTIVITY TO ELECTRICAL-STIMULATION, Journal of cardiovascular pharmacology, 23(3), 1994, pp. 453-457
Human saphenous veins were obtained at operation and assayed immediate
ly (n = 10). The veins were cut into rings, suspended in organ chamber
s, and connected to force transducers for recording of isometric tensi
on. One ring served as control; others were treated with either the al
pha(1)-adrenoceptor antagonist prazosin (Pz, 3 x 10(-7) M) or the alph
a(2)-adrenoceptor antagonist rauwolscine (Rw(1), 10(-7) M). Cooling fr
om 37 degrees to 24 degrees C had no significant effect on the resting
tone of quiescent rings. Electrical stimulation (0.2-16 Hz) caused fr
equency-dependent contractions in control vessels. The contractions we
re inhibited by Pz (p < 0.001) and by Rw (p < 0.001). In control rings
, cooling potentiated contractions evoked at all frequencies. Similar
augmentations were induced by cooling in rings treated with the alpha(
1)-antagonist Pz. In contrast, rings treated with Rw before being elec
trically stimulated showed no significant change in contractile force
when cooled. The data indicate that in the human saphenous vein, both
alpha(1)- and alpha(2)-adrenoceptors are innervated, contributing to c
ontractile response evoked by neuronal excitation. Cold augments saphe
nous vein reactivity to endogenously released norepinephrine (NE) by a
n apparent increase in the responsiveness of alpha(2)-adrenoceptors to
agonists. This relationship between temperature and adrenoceptor resp
onsiveness is consistent with the hypothesized role of alpha(2)-adreno
ceptors in cold-induced vasospasm.