This study presents a preliminary investigation of the sources of vari
ance in the measurement of ocular microtremor frequency in a normal po
pulation. When the results from both experienced and relatively inexpe
rienced operators are pooled, factors that contribute significantly to
the total variance include the measurement procedure (p < 0.001), day
-today variations within subjects (p < 0.001), and inter-subject diffe
rences (p < 0.01). Operator experience plays a role in determining the
measurement precision: the intra-subject coefficient of variation is
about 5% for a very experienced operator, and about 14% for a relative
ly inexperienced operator.