EPITHELIUM FORMATION IN THE DROSOPHILA MIDGUT DEPENDS ON THE INTERACTION OF ENDODERM AND MESODERM

The reorganization of mesenchymal cells into an epithelial sheet is a widely used morphogenetic process in metazoans. An example of such a p rocess is the formation of the Drosophila larval midgut epithelium tha t develops through a mesenchymal-epithelial transition from endodermal midgut precursors. We have studied this process in wild type and a nu mber of mutants that show defects in midgut epithelium formation. Our results indicate that the visceral mesoderm serves as a basal substrat um to which endodermal cells have to establish direct contact in order to form an epithelium. Furthermore, we have analyzed the midgut pheno type of embryos mutant for the gene shotgun, and the results suggest t hat shotgun directs adhesion between midgut epithelial cells, which is independent from the adhesion between endoderm and visceral mesoderm.