J. Auriol et al., DEVELOPMENT OF A BISPECIFIC MONOCLONAL-ANTIBODY FOR USE IN MOLECULAR HYBRIDIZATION, Journal of immunological methods, 169(1), 1994, pp. 123-133
A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas
producing monoclonal antibody to acetyl-aminofluorene with hybridomas
producing antibody against calf intestine alkaline phosphatase. The t
etradoma line established secreted immunoglobulin manifesting parental
and bispecific binding characteristics. Bispecific monoclonal antibod
y was purified and used for a one-step immunodetection assay of non-ra
dioactive DNA and RNA probes. The immunoassay developed was able to de
tect 5 pg DNA within 2 h and gave low background noise.