DEVELOPMENT OF A BISPECIFIC MONOCLONAL-ANTIBODY FOR USE IN MOLECULAR HYBRIDIZATION

Citation
J. Auriol et al., DEVELOPMENT OF A BISPECIFIC MONOCLONAL-ANTIBODY FOR USE IN MOLECULAR HYBRIDIZATION, Journal of immunological methods, 169(1), 1994, pp. 123-133
Citations number
32
Categorie Soggetti
Immunology
ISSN journal
00221759
Volume
169
Issue
1
Year of publication
1994
Pages
123 - 133
Database
ISI
SICI code
0022-1759(1994)169:1<123:DOABMF>2.0.ZU;2-O
Abstract
A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The t etradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibod y was purified and used for a one-step immunodetection assay of non-ra dioactive DNA and RNA probes. The immunoassay developed was able to de tect 5 pg DNA within 2 h and gave low background noise.