DEVELOPMENT OF A BISPECIFIC MONOCLONAL-ANTIBODY FOR USE IN MOLECULAR HYBRIDIZATION

A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The t etradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibod y was purified and used for a one-step immunodetection assay of non-ra dioactive DNA and RNA probes. The immunoassay developed was able to de tect 5 pg DNA within 2 h and gave low background noise.