SECONDARY METABOLITES IN CELL-CULTURE OF WOODY-PLANTS .3. FORMATION OF BETA-THUJAPLICIN IN CUPRESSUS-LUSITANICA CALLUS-CULTURES TREATED WITH FUNGAL ELICITORS

Authors
SAKAI K KUSABA K TSUTSUMI Y SHIRAISHI T
Citation
K. Sakai et al., SECONDARY METABOLITES IN CELL-CULTURE OF WOODY-PLANTS .3. FORMATION OF BETA-THUJAPLICIN IN CUPRESSUS-LUSITANICA CALLUS-CULTURES TREATED WITH FUNGAL ELICITORS, Mokuzai Gakkaishi, 40(1), 1994, pp. 1-5
Citations number
17
Categorie Soggetti
Materials Science, Paper & Wood
Journal title
Mokuzai Gakkaishi → ACNP
ISSN journal
00214795
Volume
40
Issue
1
Year of publication
1994
Pages
1 - 5
Database
ISI
SICI code
0021-4795(1994)40:1<1:SMICOW>2.0.ZU;2-B
Abstract
Callus cultures of Cupressus lusitanica Miller which grow fast for gym nosperm plant cells have been established and maintained for several y ears on Gamborg B5 medium supplemented with benzylaminopurine and naph thylacetic acid as growth regulators. De novo synthesis of beta-thujap licin, a heart wood constituent of many Cupressaceae species, took pla ce in the callus while being incubated for a several days after a trea tment with culture filtrates of some fungi including wood rotting ones or yeast extract solutions. This elicitation phenomenon was greatly r eproducible. The elicitor in yeast extract was partially purified and is supposed to have a molecular mass between 10 and 50 kD.