Protoplasts were prepared from the mycelia of two basidiomycetes speci
es; Pleurotus sajor-caju (Fr.) Singer and Pleurotus eryngii (jacq. ex
Fr.) Quel.. Among these protoplasts, laccase-less mutants were found i
n the regenerated dikaryotic mycelia. The parent strains having laccas
e activities with syringaldazine showed decolorization activities with
carminic acid and Remazol brilliant blue R as substrates. However, la
ccase-less mutants showed no activities with these substrates although
growth of the mutants mycelia was rather slow.