SYNTHESIS OF HOLO PARACOCCUS-DENITRIFICANS CYTOCHROME C(550) REQUIRESTARGETING TO THE PERIPLASM WHEREAS THAT OF HOLO HYDROGENOBACTER-THERMOPHILUS CYTOCHROME C(552) DOES NOT - IMPLICATIONS FOR C-TYPE CYTOCHROME BIOGENESIS

Citation
Y. Sambongi et Sj. Ferguson, SYNTHESIS OF HOLO PARACOCCUS-DENITRIFICANS CYTOCHROME C(550) REQUIRESTARGETING TO THE PERIPLASM WHEREAS THAT OF HOLO HYDROGENOBACTER-THERMOPHILUS CYTOCHROME C(552) DOES NOT - IMPLICATIONS FOR C-TYPE CYTOCHROME BIOGENESIS, FEBS letters, 340(1-2), 1994, pp. 65-70
Citations number
31
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
00145793
Volume
340
Issue
1-2
Year of publication
1994
Pages
65 - 70
Database
ISI
SICI code
0014-5793(1994)340:1-2<65:SOHPCC>2.0.ZU;2-X
Abstract
Expression from a plasmid of the complete gene, including the codons f or the N-terminal periplasmic targeting signal, for cytochrome c(550) of Paracoccus dentrificans led to the formation of the hole protein in the periplasms of both P. dentrificans and Escherichia coli. Expressi on of the gene from which the region coding for the targeting signal h ad been specifically deleted resulted in formation of ape-protein in t he cytoplasms of both organisms. These findings are consistent with ha em attachment occurring in the periplasm. In contrast, the formation o f holo cytochrome c(552) from Hydrogenobacter thermophilus following e xpression of the gene lacking the periplasmic targeting sequence in ei ther P. denitrificans or E. coli is attributed to spontaneous cytoplas mic attachment of haem to the thermostable protein.