REACTIVITY OF PROTEIN SULFHYDRYL-GROUPS WITH DISULFIDES

Authors
PETACH HH
Citation
Hh. Petach, REACTIVITY OF PROTEIN SULFHYDRYL-GROUPS WITH DISULFIDES, Tetrahedron letters, 35(10), 1994, pp. 1587-1588
Citations number
7
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
35
Issue
10
Year of publication
1994
Pages
1587 - 1588
Database
ISI
SICI code
0040-4039(1994)35:10<1587:ROPSWD>2.0.ZU;2-H
Abstract
The detection of sulfhydryl groups in proteins is limited by the inacc essibility of the sulfhydryls to the reagent, 5,5'-dithiobis(2-nitrobe nzoic acid). The addition of other disulfides such as 2,2'-dithiobis(e thylamine) to the chromophoric disulfide increases both the rate and t he extent of detection of ''buried'' sulfhydryl groups in proteins.