CHROMOSOMAL MAPPING OF MOUSE GENES EXPRESSED SELECTIVELY WITHIN THE CENTRAL-NERVOUS-SYSTEM

We have used RFLP analysis on DNA from a panel of interspecific (C57BL /6J x Mus spretus) F-1 x M. spretus backcross offspring to assign the genes encoding 10 neuron-specific mRNAs and 2 loci corresponding to cy clophilin a-related sequences to the mouse chromosomal map. The Pss1 l ocus encoding the forebrain-enriched protein kinase C substrate RC3, a component of dendritic spines, mapped to proximal Chr 9. The Camkl lo cus encoding the calmodulin-binding protein kinase-like vesicle protei n 1G5 mapped to distal Chr 9. The Gng7 locus encoding the gamma 7 G-pr otein subunit, highly enriched in the striatum and presumptively coupl ed to dopamine receptors, mapped to mid-Chr 10. The Htr1f, Htr5a, Htr5 b, and Htr7 loci, encoding four serotonin receptors, mapped to Chr 16, 5, 1, and 19, respectively. The Peplb locus, encoding a CD26 ectopept idase-like neuronal membrane protein activated by kainate and long-ter m potentiation, mapped to Chr 5. The D2Sutle and Cpu3 loci, encoding n eural proteins of unknown functions, mapped to Chrs 2 and 9, respectiv ely. Two cyclophilin 2-related loci, Cphn2-r1 and Cphn2-r2, mapped to different regions of Chr 9. Comparison of these 12 newly mapped loci w ith the existing mouse map and known regions of syntenic homology with the human map, along with the known features and expression profiles of the products of these genes, suggests a few candidates for mouse mu tations and human neurological and immunological deficits, including t he Tourette syndrome and Bloom syndrome genes. (C) 1994 Academic Press , Inc.