CLONING OF CDNA AND GENOMIC DNA ENCODING HUMAN TYPE-XVIII COLLAGEN AND LOCALIZATION OF THE ALPHA-1(XVIII) COLLAGEN GENE TO MOUSE CHROMOSOME-10 AND HUMAN-CHROMOSOME-21

Types XV and XVIII collagen belong to a unique and novel subclass of t he collagen superfamily for which we have proposed the name the MULTIP LEXIN family. Members of this class contain polypeptides with multiple triple-helical domains separated and flanked by non-triple-helical re gions. In this paper, we report the isolation of human cDNAs and genom ic DNAs encoding the alpha 1(XVIII) collagen chain. Utilizing a genomi c clone as probe, we have mapped the COL18A1 gene to chromosome 21q22. 3 by fluorescence in situ hybridization. In addition, using an intersp ecific backcross panel, we have shown that the murine Col18al locus is on chromosome 10, close to the loci for Col6a1 and Col6a2. (C) 1994 A cademic Press, Inc.